OSM(OncostatinM)isasecretedcytokineinvolvedinhomeostasisandmostchronicillnesses.OSM,amemberoftheIL-6(gp130)familyofproteins,inhibitstheproliferationofanumberoftumorcelllinesandplaysasignificantroleinhematopoiesis,mesenchymalstemcelldifferentiation,liverregeneration,heartremodeling,nociception,inflammationandmetabolism.OfallIL-6-typecytokinesstudiedsofar,OSMdisplaysthebroadestsignalingprofile.LIFandOSMproteinsactivatetheJAK/STATpathway,theMAPKproteinkinasesERK1andERK2,thestress-activatedproteinkinasesp38andc-JunN-terminalkinase(JNK),thephosphatidylinositol-3-kinase(PI3K)AKTpathwayandproteinkinaseCdelta(PRKCD).Inaddition,OSMexhibitsmanyothereffectsbyregulatingtheproductionofothercytokinesincludingIL-6,G-CSFandGM-CSFinendothelialcells.OSMmaybeatumorpromoterthatwillprovideapotentialtargetforantiangiogenictherapy.TheBovineOncostatinM(OSM)Do-It-Yourself
ELISAcontainscaptureantibody,proteinstandard,anddetectionantibodyfordevelopmentofaBovineOncostatinM(OSM)ELISA.TheantibodieshavebeendeterminedtofunctioninanELISAwiththestandardprovided.Optimalbuffers,concentrations,incubationtimes,incubationtemperatures,andmethodsfortheELISAhavenotbeendetermined.AworkingknowledgeofELISAisstronglyrecommended. Thequantitiesofcomponentsprovidedarenotmatched.Componentsmayalsobepurchasedseparately.
| Component | Usage | Quantity | Catalog# |
| Anti-BovineOncostatinM(OSM) PolyclonalAntibody | CaptureAntibody | 100µg | KP1362B-100 |
| BiotinylatedAnti-BovineOncostatinM(OSM) PolyclonalAntibody | DetectionAntibody | 50µg | KPB1363B-050 |
| BovineOncostatinM(OSM)RecombinantProtein | Standard | 5µg | RP1182B-005 |