IL-12isamemberoftheIL-12family,whichincludesIL-12,IL-23,IL-27,andIL-35.LikeotherIL-12familymembers,IL-12isaheterodimericcytokine.IL-12isencodedbytwoseparategenes,IL-12A(p35)andIL-12B(p40).Theactiveheterodimer,andahomodimerofp40areformedfollowingproteinsynthesis.IL-12isinvolvedinthedifferentiationofnaiveTcellsintoTh1cells.ItisknownasaTcell-stimulatingfactor,whichcanstimulatethegrowthandfunctionofTcells.ItstimulatestheproductionofIFN-γandTNF-αfromTandnaturalkiller(NK)cells,andreducesIL-4mediatedsuppressionofIFN-γ.
TheEquineIL-12(IL-23)p40 Do-It-Yourself
ELISAcontainscaptureantibody,standard,anddetectionantibodyfordevelopmentofaEquineIL-12(IL-23)p40 ELISA.TheantibodieshavebeendeterminedtofunctioninanELISAwiththestandardprovided.Optimalbuffers,concentrations,incubationtimes,incubationtemperatures,andmethodsfortheELISAhavenotbeendetermined.AworkingknowledgeofELISAisstronglyrecommended. Thequantitiesofcomponentsprovidedarenotmatched.Componentsmayalsobepurchasedseparately.
| Component | Usage | Quantity | Catalog# |
| Anti-EquineIL-12(IL-23)p40PolyclonalAntibody | CaptureAntibody | 100µg | KP1225E-100 |
| BiotinylatedAnti-EquineIL-12(IL-23)p40PolyclonalAntibody | DetectionAntibody | 50µg | KPB1226E-050 |
| EquineIL-12(IL-23)p40RecombinantProtein | Standard | 5µg | RP0924E-005 |