Interleukin-6 (IL-6)isaninterleukinthatactsasbothapro-inflammatoryandanti-inflammatorycytokine.ItissecretedbyTcellsandmacrophagestostimulateimmuneresponsetotrauma,especiallyburnsorothertissuedamageleADIngtoinflammation.IL-6isalsoproducedfrommuscle,andiselevatedinresponsetomusclecontraction.Itissignificantlyelevatedwithexercise,andprecedestheappearanceofothercytokinesinthecirculation. OsteoblastssecreteIL-6tostimulateosteoclastformation.SmoothmusclecellsinthetunicamediaofmanybloodvesselsalsoproduceIL-6asapro-inflammatorycytokine.TheroleofIL-6asananti-inflammatorycytokineismediatedthroughitsinhibitoryeffectsonTNF-alphaandIL-1,andactivationofIL-1raandIL-10.TheRabbitIL-6Do-It-Yourself
ELISAcontainscaptureantibody,standard,anddetectionantibodyfordevelopmentofaRabbitIL-6ELISA.TheantibodieshavebeendeterminedtofunctioninanELISAwiththestandardprovided.Optimalbuffers,concentrations,incubationtimes,incubationtemperatures,andmethodsfortheELISAhavenotbeendetermined.AworkingknowledgeofELISAisstronglyrecommended. Thequantitiesofcomponentsprovidedarenotmatched.Componentsmayalsobepurchasedseparately.
| Component | Usage | Quantity | Catalog# |
| Anti-RabbitIL-6PAb | CaptureAntibody | 100µg | KP1390U-100 |
| BiotinylatedAnti-RabbitIL-6PAb | DetectionAntibody | 50µg | KPB1391U-050 |
| RabbitIL-6Protein | Standard | 5µg | RP1281U-005 |